Differential Effects of Low-Dose Erythropoietin in Rat Model of Diabetic Nephropathy Submitted: Jan 3, 2018 Accepted: Feb 26, 2018 Published online: Mar 3, 2018


Abdel-Motaal Fouda Rehab H. Ashour Farida El-Banna Mohamed A. Saad Fatma A. Mostafa Manal I. Fouda


Background. Previous reports on the renoprotective effect of erythropoietin (EPO) in the setting of chronic kidney disease (CKD) have yielded conflicting results. The aim of this study is to clarify the effect of low, non-hematopoietic dose of EPO on the evolution of diabetic nephropathy (DN) in rat model. Methods. Low dose of recombinant human EPO (150 U/kg, s.c. three times/week) was given to streptozotocin (STZ)-induced diabetic rats in two schedules; in the first one, EPO was given from day 2 after STZ injection till the end of the study (28 weeks) as prophylactic treatment; and in the other schedule EPO was given after development of DN (last 8 weeks) as therapeutic treatment. Albuminuria, blood pressure, creatinine clearance, renal venous oxygen tension (vPO2), plasma EPO, hematocrit and renal histopathology were assessed. Results. Unexpectedly, 28 weeks administration of EPO to diabetic rats led to aggravation of albuminuria and worsening of histopathological damage in spite of partial correction of renal hypoxia. Contrary to this, terminal 8 weeks EPO therapy of DN reduced albuminuria and demonstrated some favorable effects on biochemical changes and histologic picture. Conclusion. Low dose EPO exerted differential effects in rat model of DN according to treatment duration. In addition, findings of the present study warrants further investigations of the exact renoprotective role of EPO in diabetic patients with CKD who receive EPO therapy for long periods.


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Erythropoietin, Diabetic nephropathy, Renal hypoxia, Albuminuria

How to Cite
Fouda, A.-M. ., Ashour, R. H. ., El-Banna, F. ., Saad, M. A. ., Mostafa, F. A. ., & Fouda, M. I. . (2018). Differential Effects of Low-Dose Erythropoietin in Rat Model of Diabetic Nephropathy: Submitted: Jan 3, 2018 Accepted: Feb 26, 2018 Published online: Mar 3, 2018. Advances in Medicine and Medical Research, 1(1), 25–33. https://doi.org/10.31377/ammr.v1i1.494
Original Articles
Author Biography

Abdel-Motaal Fouda, Department of Clinical Pharmacology, Mansoura University Faculty of Medicine, Mansoura 35516, Egypt


Corresponding Author:

Email: foudaamm@mans.edu.eg Tel: 00201113974766


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